Enhanced Sensitivity Using MALDI Imaging Coupled with Laser Postionization (MALDI-2) for Pharmaceutical Research

Imaging with ion beams and laser postionization.

We investigate the use of a liquid metal ion gun (LMIG) combined with multiphoton resonance ionization of sputtered atoms and/or molecules to acquire mass-selected images with submicrometer spatial resolution. Images from several model systems are presented including those of an In grid, and of patterned surfaces of benzo[a]pyrene and tryptophan thin films. Since postionization for these system...

Selected MALDI Imaging Publications

Early lipid changes in acute kidney injury using SWATH lipidomics coupled with MALDI tissue imaging.

Acute kidney injury (AKI) is one of the leading causes of in-hospital morbidity and mortality, particularly in critically ill patients. Although our understanding of AKI at the molecular level remains limited due to its complex pathophysiology, recent advances in both quantitative and spatial mass spectrometric approaches offer new opportunities to assess the significance of renal metabolomic c...

Developing MALDI Imaging Technology for Cancer Profile

Normal and diseased tissues are complex mixtures of different cell populations. A better understanding of protein expression changes that occur during 
diseases needs sensitive and specific technologies for each of these cell types. Mass spectrometry based tissue imaging (MALDI-MSI) is a newly developed technique, allowing the visualization of proteins, peptides, lipids and small molecules dire...

MALDI Tissue Imaging of Drugs with the ultraflex MALDI TOF/TOF

MALDI-TOF MS has previously been used for imaging of large peptides and proteins embedded in tissue sections. This imaging technique is not limited to larger molecules, but can also be used to measure the presence of small molecules directly from tissue. However, for small molecule imaging the method of choice is a selected reaction monitoring approach in MS/MS mode.The benefits of parent ion i...